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Disease Pharmacology

We Offer Specialized and Standard Disease and Pharmacology Models

 

Hypertension and Blood Pressure

    • Radio-Telemetry – Continuous measurement of blood pressure, heart rate, ECG, respiration, activity and temperature in conscious, unrestrained animals for up to 6 months
    • Tethered Model – Continuous measurement of blood pressure with simultaneous infusion of test compound along with blood, urine and/or fecal collection for up to 10 days

Species: Rat, Mouse and Guinea Pig

Disease Models: SHR, Dahl Salt Sensitive (DSS), ZSF1, ANGII-induced (additional models available)

 

Nephropathy

    • Proteinuria, albuminuria, GFR, Creatinine Clearance, histological evaluation
    • Plasma/Urine Biomarkers of damage
    • Chronic blood pressure monitoring using radio telemetry

Disease Models: Uninephrectomized, Dahl Salt Sensitive (DSS), Streptozotocin (STZ), ZSF1, Unilateral Urethral Obstruction (UUO), Puromycin Aminonucleoside (PAN) (additional models available)

 

Heart Failure

    • Left Ventricular Function, Cardiac remodeling and Histological evaluation

Methods: Echocardiography (M-Mode, B-Mode & Doppler), Pressure/Volume, Invastive LV Hemodynamics (+/-dP/dT, Tau, LVEDP and LVESP)

Disease Models: Myocardial Infarction, ZSF1, SHHF, Dahl Salt Sensitive (DSS) (additional models available)

 

Fibrosis

    • Collagen/Hydroxyproline content, functional endpoints (e.g. liver enzymes, creatinine) and Histological evaluation

Models: Bile Duct Ligation (BDL) and Unilateral Ureteral Obstruction (UUO)

 

Pulmonary Arterial Hypertension

    • Right Ventricular Systolic Pressure/Pulmonary Arterial Pressure
    • Heart and Lung Weight
    • Myocardial, Pulmonary Artery and Lung Histology

Disease Models: Monocrotaline PAH

 

Metabolic

    • Glucose and Insulin Tolerance Tests (OGTT/IPGTT)
    • Fasting and fed blood glucose
    • Cholesterol, HDL, LDL, Apolipoproteins, HbA1C, Microalbumin, Creatinine, etc.
    • Adipose tissue and body weight

Disease Models: Type I and II diabetes – ob/ob, db/db, DIO and STZ mice; ZDF and STZ rats (additional models available)

 

Gastrointestinal

    • Infectious Diarrhea – Rotavirus-induced diarrhea
    • Intestinal Secretion – Open and closed loop models of secretion induced by cholera toxin or ETEC (LT/ST)
    • Stool Output – Castor oil-induced diarrhea model
    • Intestinal Motility – Charcoal meal test

 

Inflammation

    • Carrageenan, LPS, Strep Cell Wall or Zymosan induced inflammation
    • Inflammatory cell counts, PG/LT assay, exudate volume and edema

Disease Models: Air Pouch, Paw Edema and Systemic

 

Microscopy & Histology

    • We offer a wide range of microscopy and histology services to enable detailed quantitative organ/tissue analysis and morphometry
    • Fixation and processing of samples into paraffin, OCT and plastic
    • Sectioning and staining of tissue blocks for LM histology and EM ultrastructure
    • Range of quantitative microscopy techniques including; Epi-fluorescence, Laser scanning & capture, Electron microscopy